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Confocal Raman microscopy with the EasyConfocal™ method
The inVia microscope uses the EasyConfocal™ method to be highly confocal. This method is an efficient way to spatially filter out-of-focus and off-axis light. It gives you excellent confocal performance without a hard-to-align pinhole. The EasyConfocal method is a proven way of producing 1D profiles, 2D images and 3D volumes at high spatial resolution.
Confocal Raman microscopes restrict the sampling volume by placing an aperture at a suitable point before the detector. The aperture rejects out-of-focus and off-axis light from outside the sampling volume. You can control the degree of confocality by choosing different the microscope optics. To achieve a high degree of confocality, you should use high-magnification objectives and small aperture sizes.
Why use confocal Raman microscopy?
Confocal Raman microscopy can give information about depth and inclusions structures within optically transparent samples. Confocal microscopy enables the highest spatial resolution when analysing chemical and structural information in up to three dimensions.
| Inclusion in a matrix | Laminates and thin layers |
| • Active drug in an excipient • Carbon fibre in epoxy resin • Fluid and solid inclusion in geological sections and gem stones • Semiconductor defect analysis | • Thin polymer films • Sectioned biomaterials • Semiconductor heterostructure junction |
Advantages of the EasyConfocal method
The EasyConfocal method removes the need for a classical pinhole to achieve high spatial resolution. This method combines two apertures: one physical (the spectrograph entrance slit) and one defined in software (the height of the captured image on the CCD). This is an effective spatial filter for the scattered light and gives excellent spatial resolution. Using this approach, we have shown data quality as good as that of a pinhole-based microscope.
Measurements on the inVia™ confocal Raman microscope use the EasyConfocal method. This has significant advantages over a traditional pinhole. By controlling the spectrometer slit and the CCD area, the instrument maintains full confocal performance throughout the UV-visible range. There is no need to realign or refocus the pinholes, as used in older system designs.
Easy confocal Raman configuration
With the EasyConfocal method, you have easy of control the confocal sampling volume of a Raman measurement.
• High confocal mode for cell volume mapping, or polymer laminate layer profiling
• Standard confocal mode for most mapping and analysis experiments
These options are simple to configure; just select the setting you want in the software. You can also start an automated routine to optimise throughput with just a single click in the software.
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Technical note: Renishaw’s EasyConfocal™ Raman microscope method
This note explains how Renishaw’s Raman microscopes have been designed to offer users the best confocal performance without sacrificing efficiency or ease of use.