細胞生物學
拉曼光譜為非侵入性、無標籤的技術。擷取化學資訊,無須操縱基因,或使用著色/抗體。有助於確保結果反映真正的細胞化學。
識別細胞
使用 Renishaw 拉曼系統識別和分辨:
- 癌細胞與正常細胞
- 幹細胞與分化細胞
- 細胞群中的不同子狀態 (如幹細胞和前驅細胞)
您可以根據細胞本身的化學性剖面,識別無已知標記的細胞。完全無須與抗體共軛或操縱基因。
查看微小生物細節
藉由高空間解析度共軛焦拉曼分析,可檢驗:
- 現場檢驗個別細胞的胞內結構和生物分子
- 酵母細胞化學含量
- 癌細胞的脂質含量,以更加瞭解脂質代謝
研究細胞群內的個別細胞並判斷細胞間變異性。例如,您可以分析健康細胞和異常細胞的脂質分佈和 DNA。
研究活細胞
您可以在 Renishaw 拉曼系統上配備細胞培養箱。此配件可控制溫度、CO2 濃度和濕度,讓細胞在分析期間維持正常生理狀態。
比起末端實驗,活細胞資料更能呈現任何動態過程。例如,您可以監控細胞對環境或藥物變化的反應。這些反應可能會以代謝/形態變化或細胞死亡 (凋亡) 呈現,而這些都可透過拉曼光譜偵測。
以 3D 視圖解析胞內變化
收集化學資訊並產生樣品的 3D 視圖,利用這些視圖驗證個別細胞的攝入物質。您也可以判斷細胞量及其胞器。
下載:生命科學(細胞)
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Application note: Classification of brain glioma tumours using the Renishaw Biological Analyser [en]
Demonstrating discrimination between diseased and healthy brain tissue using the Renishaw RA816 Biological Analyser.
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Application note: Cell imaging with the inVia confocal Raman microscope [en]
With Renishaw’s inVia confocal Raman microscope you can identify and characterise samples to provide chemical, spatial and structural information on multiple types of molecule, without labelling. It provides rich, detailed, chemical images and highly specific data at high spatial resolution, making it ideal for studying cells.
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Application note: Surface enhanced Raman spectroscopy (SERS) imaging using the inVia confocal Raman microscope [en]
Raman imaging is a powerful research tool for understanding the molecular composition, structure and distribution of different chemical species. Nano silver/gold colloids and roughened metallic substrates can be used to amplify the intensity of the Raman scattering of adsorbed molecules via SERS. This can increase the sensitivity and/or the specificity of the analysis. SERS imaging can be used to evaluate the efficacy of delivery of nanoparticles (NPs) into cells/animals. SERS measurement of labelled or surface-modified NPs can also be used for biosensing, multiplexing and theranostics.
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Application note: Raman imaging to reveal components and metabolites in wood cells and tissue [en]
Analysing Scots pine wood using the inVia™ confocal Raman microscope, to reveal high-resolution details of structure and chemical composition.
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Portrait of a dying cell [en]
The December 2015 issue of 'The Pathologist' featured an article describing how Raman spectroscopy is a non-invasive way of obtaining morphological and chemical information about cells that may lead to better cancer research.
以下為您可能感興趣的論文:
Lau et al (2014) Biomedical Spectroscopy and Imaging 3: 237-247
McAughtrie et al (2013) Chem Sci 4: 3566-72
Kim et al (2010) Anal Bioanal Chem 398: 3051-3061
最新細胞生物學消息
inVia 用於研究塑膠血袋之中存放的血液
加拿大溫哥華英屬哥倫比亞大學 (University of British Columbia) 的 Michael Smith 實驗室,率先使用拉曼光譜,針對存放的紅血球 (RBC) 單位1,2 監控生物化學變化,以及捐贈者之間的變異性。由 Michael Blades 及 Robin Turner 教授組成的研究小組,最近於 Analyst 期刊發表此項研究成果。
拉曼光譜用於在癌症治療期間偵測細胞及組織的輻射損傷
加拿大英屬哥倫比亞大學 (University of British Columbia) 的 Irving K Barber 文理學院 (Irving K Barber School of Arts and Sciences),擁有由物理、工程及放射腫瘤科學家組成的多學科小組。該小組致力於偵測及瞭解癌症治療期間,使用離子化輻射對細胞及組織造成的損傷。